Identification of Phytoplasmas on Carrot (Daucus carota L.) and Leafhopper Associated with Yellow Disease in Bogor and Bandung, West Java
نویسندگان
چکیده
Gejala kuning mirip seperti infeksi fitoplasma ditemukan pada pertanaman wortel di Jawa Barat. Penelitian bertujuan mengidentifikasi tanaman dan serangga wereng yang berasosiasi dengan gejala Bogor Bandung. Wereng terdiri atas 5 spesies daun (Hemiptera: Cicadellidae), yaitu Balclutha incisa (Matsumura), Orosius argentatus (Evans), Cicadulina bipunctata Empoascanara indica (Datta), Exitianus indicus (Distant), 1 batang Delphacidae), Sogatella furcifera (Horváth). Deteksi dilakukan nested-PCR, sikuensing, analisis DNA sikuen. Nested-PCR berhasil mengamplifikasi target, gen 16S rRNA dari wereng. Analisis identitas matriks sikuen asal menunjukkan homologi tertinggi grup 16SrII, Peanut witches’-broom phytoplasma; sedangkan sampel Bandung 16SrI, Ca. Phytoplasma asteris Tomato big bud phytoplasma. (B. incisa, S. furcifera) Cactus C. asteris. Tulisan ini merupakan laporan pertama menginfeksi
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ژورنال
عنوان ژورنال: Jurnal Fitopatologi
سال: 2022
ISSN: ['2339-2479', '0215-7950']
DOI: https://doi.org/10.14692/jfi.16.4.157-165